What You Need to Know
When heated to 85–90 °C, agarose dissolves and aligns into double‑helical junction zones. Cooling to 32–40 °C locks these junctions together, creating a polysaccharide network that can withstand temperatures up to 80 °C. Gel strength scales with concentration and is modulated by ionic strength and additives.
The Science
Primary Reaction
Thermoreversible gelation of agarose via double‑helical junction formation