The Science
Primary Reaction
Enzyme-catalyzed hydrolysis
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Key Variables
Temperature (e.g., 55°C for transglutaminase, 60-70°C for amylase)pH (e.g., 6.0-7.0 for most enzymes)Enzyme concentration (e.g., 0.1-1.0% w/w)Substrate concentration (e.g., 1-10% w/w)Reaction time (e.g., 30 minutes to several hours)
Common Mistakes
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Enzyme denaturation
Cause: Exceeding optimal temperature or pH range
Fix: Adjust temperature or pH to optimal range