What You Need to Know
CRISPR‑Cas9 introduces targeted double‑strand breaks that are repaired via homology‑directed repair or non‑homologous end joining, while RNAi constructs produce small interfering RNAs that guide sequence‑specific mRNA degradation. Transgenic insertion relies on Agrobacterium‑mediated T‑DNA integration, generating stable transgene expression under promoter control.
The Science
Primary Reaction
CRISPR‑Cas9 mediated double‑strand break and repair via homology‑directed repair or non‑homologous end joining.