What You Need to Know
The CRISPR‑Cas9 system introduces double‑strand breaks that are repaired by the cell’s homologous recombination or non‑homologous end joining pathways, while Agrobacterium transfers a T‑DNA cassette into the plant genome via a type IV secretion system. Both approaches rely on tightly controlled temperature, hormone, and incubation parameters to maximize integration efficiency and minimize unintended mutations.
The Science
Primary Reaction
CRISPR‑Cas9 mediated double‑strand break repair and Agrobacterium‑mediated T‑DNA integration