What You Need to Know
The process relied on proteolytic enzymes and halophilic bacteria to hydrolyze fish proteins into amino acids, peptides, and volatile esters. The resulting liquid contained high levels of glutamic acid, tyramine, histamine, and characteristic esters such as 3‑methyl‑2‑butenyl‑1‑yl acetate, giving it a pungent, umami-rich aroma.
The Science
Primary Reaction
Proteolysis and fermentation by halophilic bacteria