Method for the Determination of the Free Fatty Acids of Milk Fat
Donald D. Bills, Lakho Lilaram Khatri, E.A. Day
Journal of Dairy Science
Abstract
Determination of the free fatty acids of milk fat by means of gas-liquid chromatography, employing two internal standards was evaluated. The free fatty acids were isolated from the fat by means of a basic anion exchange resin, converted to methyl esters, and extracted with ethyl chloride. The ethyl chloride solution of methyl esters was then concentrated with a special reflux systern prior to chromatography to prevent the loss of the more volatile esters. Appropriate factors were calculated for relating the quantity of added internal standards to that of the naturally occurring free fatty acids.
Extracted Claims
3 claims extracted from this paper into the knowledge graph
basic anion exchange resin used for isolation of free fatty acids
“The free fatty acids were isolated from the fat by means of a basic anion exchange resin, converted to methyl esters, and extracted with ethyl chloride.”
reflux system used for concentration of ethyl chloride solution
“The ethyl chloride solution of methyl esters was then concentrated with a special reflux systern prior to chromatography to prevent the loss of the more volatile esters.”
gas-liquid chromatography used for determination of free fatty acids in milk fat
“Determination of the free fatty acids of milk fat by means of gas-liquid chromatography, employing two internal standards was evaluated.”