Tofu

“Approximately 50% of the marker can then be enzymatically released by incubation with a cell-free extract from fresh yeast.”

β-fructofuranosidase → is released by → incubation with a cell-free extract from fresh yeast

“We also solved the 1.8 Å crystal structure of the β-fructofuranosidase complex with β-D-fructose, a hydrolysis product obtained by soaking apo crystal in raffinose.”

crystal structure → resolved → β-fructofuranosidase complex with β-D-fructose

“This enzyme hydrolyzed sucrose, erlose, raffinose, fructosylxyloside, neokestose, and stachyose at the relative velocities of 100, 75, 61, 59, 54, and 11, but hardly hydrolyzed 1-kestose and nystose (less than 0.02).”

beta-fructofuranosidase I → hydrolyzes → sucrose, erlose, raffinose, fructosylxyloside, neokestose, and stachyose

“The enzyme hydrolyzed sucrose with a Km of 5 mM and a broad pH optimum around 5.0.”

beta-fructofuranosidase → hydrolyzes → sucrose

“The purified protein, which was N-glycosylated with high-mannose-containing and high-xylose-containing complex glycans, eluted as a monomeric polypeptide with a molecular mass of 68,000 from a gel-filtration column.”

beta-fructofuranosidase → elutes as → monomeric polypeptide